CHROMOSOME MAPPING IN SACCHAROMYCES: CENTROMERE-LINKED GENES
نویسندگان
چکیده
منابع مشابه
The Use of Linear Asci for Chromosome Mapping in Saccharomyces.
N yeast, as in other organisms where tetrad analysis is feasible, it is possible to Idetermine the distance between a given gene and the centromere of its chromosome. This may be accomplished directly if the relationship of the spores in the ascus is known. Two such relationships have been demonstrated. In the four-spored asci of Schizosaccharomyces pombe, the two spores at each end of the ascu...
متن کاملIdentification and characterization of the centromere from chromosome XIV in Saccharomyces cerevisiae.
A functional centromere located on a small DNA restriction fragment from Saccharomyces cerevisiae was identified as CEN14 by integrating centromere-adjacent DNA plus the URA3 gene by homologous recombination into the yeast genome and then by localizing the URA3 gene to chromosome XIV by standard tetrad analysis. DNA sequence analysis revealed that CEN14 possesses sequences (elements I, II, and ...
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We have purified two new complexes from Saccharomyces cerevisiae, one containing the centromere component Mtw1p together with Nnf1p, Nsl1p, and Dsn1p, which we call the Mtw1p complex, and the other containing Spc105p and Ydr532p, which we call the Spc105p complex. Further purifications using Dsn1p tagged with protein A show, in addition to the other components of the Mtw1p complex, the two comp...
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The organization of chromosomes into territories plays an important role in a wide range of cellular processes, including gene expression, transcription, and DNA repair. Current understanding has largely excluded the spatiotemporal dynamic fluctuations of the chromatin polymer. We combine in vivo chromatin motion analysis with mathematical modeling to elucidate the physical properties that unde...
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The centromere is an essential chromosomal structure that is required for the faithful distribution of replicated chromosomes to daughter cells. Defects in the centromere can compromise the stability of chromosomes resulting in segregation errors. We have characterised the centromeric structure of the spontaneous mutant mouse strain, BALB/cWt, which exhibits a high rate of Y chromosome instabil...
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ژورنال
عنوان ژورنال: Genetics
سال: 1960
ISSN: 1943-2631
DOI: 10.1093/genetics/45.8.1085